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Abstract. Histone deacetylases (HDACs) are best known as transcription corepressors through deacetylating histone tails. Here we show that HDAC2 is also involved in cap-dependent mRNA translation by promoting sumoylation of eukaryotic translation initiation factor 4E (eIF4E), which is independent of its deacetylase activity.
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Aug 26, 2021 · Experiment type. Expression profiling by high throughput sequencing. Summary. Immune responses to cancer are highly variable, in particular colorectal cancer tumors with mismatch repair-deficiency (MMRd) show an elevated presence and activity of immune cells as compared to mismatch repair proficient tumors.
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Aug 26, 2021 · The cell suspension was spun at 500 g for 7 min in a 4 ̊C pre-cooled centrifuge to pellet the cells. The pellet was lysed in 4ml ammonium-chloride-potassium (ACK) buffer for 2 minutes and then stopped with RPMI containing 2% human serum. The cell suspension was then centrifuged at 500 g for 7 min at 4 ̊C.
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